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PUBLICATIONS 2010 PROTEIN CRYSTALLOGRAPHY
Rouvinen J, Jänis J, Laukkanen M-L, Jylhä S, Niemi M, Päivinen T, Mäkinen-Kiljunen S, Haahtela T, Söderlund H & Takkinen K: Transient dimers of allergens. PLoS ONE 5 (2010) e9037. Niemi MH, Takkinen K, Amundsen L, Söderlund H, Rouvinen J & Höyhtyä M: The testosterone binding mechanism of an antibody derived from a naïve human scFv library. J. Mol. Recog. in press. 2010 PROTEIN MASS SPECTROMETRY Anbarasan S, Jänis J, Paloheimo M, Laitaoja M, Vuolanto M, Karimäki J, Vainiotalo P, Leisola M & Turunen O: The effect of glycosylation and additional domains on the thermostability of a family 10 xylanase of Thermopolyspora flexuosa. Appl. Environ. Microbiol. 76 (2010) 356-360. 2009 PROTEIN CRYSTALLOGRAPHY
Kallio JM, Hakulinen N, Kallio JP, Niemi MH, Kärkkäinen S & Rouvinen J: The contribution of polystyrene nanospheres towards the crystallization of proteins. PLoS ONE 4 (2009) e4198. Kallio JP, Auer S, Jänis J, Andberg M, Kruus K, Rouvinen J, Koivula A & Hakulinen N: Structure-function studies of a Melanocarpus albomyces laccase suggest a pathway for oxidation of phenolic compounds. J. Mol. Biol. 392 (2009) 895-909. Maksimainen M, Timoharju T, Kallio JM, Hakulinen N, Turunen O & Rouvinen J: Crystallization and preliminary diffraction analysis of b-galactosidase from Trichoderma reesei. Acta Cryst. F 65 (2009) 767-769. Kallio JP, Jänis J, Nyyssölä A, Hakulinen N & Rouvinen J: Preliminary X-ray analysis of twinned crystals of a sarcosine dimethylglycine methyltransferase from Halorhodospira halochoris. Acta Cryst. F 65 (2009) 805-808. Andberg M, Hakulinen N, Auer S, Saloheimo M, Koivula A, Rouvinen J & Kruus K: Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure. FEBS J. 276 (2009) 6285-6300.
2009 PROTEIN MASS SPECTROMETRY
Voutilainen SP, Boer H, Alapuranen M, Jänis J, Vehmaanperä, J & Koivula, A: Improving the thermostability and activity of Melanocarpus albomyces cellobiohydrolase Cel7B. Appl. Microbiol. Biotechnol. 83 (2009) 261–272.
Viiri KM, Jänis J, Siggers T, Heinonen TY, Valjakka J, Bulyk ML, Mäki M & Lohi O: DNA-binding and -bending activities of SAP30L and SAP30 are mediated by a novel zinc-dependent structure and monophosphoinositides. Mol. Cell. Biol. 29 (2009) 342-356.
2008 PROTEIN CRYSTALLOGRAPHY
Niemi M, Jänis J, Jylhä S, Kallio JM, Hakulinen N, Laukkanen M-L, Takkinen K & Rouvinen J: Characterization and crystallization of a recombinant IgE Fab fragment in complex with the bovine b-lactoglobulin. Acta Cryst F 64 (2008) 25-28. Saarelainen S, Rytkönen-Nissinen M, Rouvinen J, Taivainen A, Auriola S, Kauppinen A, Kinnunen T & Virtanen T: Animal-derived lipocalin allergens exhibit IgE cross-reactivity. Clin. Exp. Allergy 38 (2008) 364-381. Hakulinen N, Andberg M, Kallio J, Koivula A, Kruus K & Rouvinen J: A near-atomic resolution structure of a Melanocapus albomyces laccase. J. Struct. Biol. 162 (2008) 39-39. Parkkinen T, Koivula A, Vehmaanperä J & Rouvinen J: Crystal structures of Melanocarpus albomyces cellobiohydrolase Cel7B in complex with cello-oligomers show high flexibility in the substrate binding. Protein Sci.17 (2008) 1383-1394. 2008 PROTEIN MASS SPECTROMETRY
Jänis J, Rouvinen J, Vainiotalo P, Turunen O & Shnyrov VL: Irreversible thermal denaturation of Trichoderma reesei endo-1,4-b-xylanase II and its three disulfide mutants characterized by differential scanning calorimetry and ESIFT-ICR mass spectrometry. Int. J. Biol. Macromol. 42 (2008) 75-80. Jänis J, Pasanen S, Rouvinen J & Vainiotalo P: Characterization of a pH dependent dissociation of Streptomyces rubiginosus xylose isomerase by ESI FT-ICR mass spectrometry. J. Mass Spectrom.43 (2008) 1376-1380. Bootorabi F, Jänis J, Valjakka J, Isoniemi S, Vainiotalo P, Vullo D, Supuran CT, Waheed A, Sly WS, Niemelä O & Parkkila S: Modification of carbonic anhydrase II with acetaldehyde, the first metabolite of ethanol, leads to decreased enzyme activity. BMC Biochem. (2008) 9:32. Hilvo M, Baranauskiene L, Salzano AM, Scaloni A, Matulis D, Innocenti A, Scozzafava A, Monti SM, Di Fiore A, De Simone G, Lindfors M, Jänis J, Valjakka J, Pastoreková S, Pastorek J, Kulomaa MS, Nordlund HR, Supuran CT & Parkkila S: Biochemical characterization of CA IX: One of the most active carbonic anhydrase isozymes. J. Biol. Chem. 283 (2008) 27799-27809. Helppolainen SH, Määttä JAE, Halling KK, Slotte JP, Hytönen VP, Jänis J, Vainiotalo P, Kulomaa MS & Nordlund HR: Bradavidin II from Bradyrhizobium japonicum; A new avidin-like biotin-binding protein. BBA Proteins Proteom. 1784 (2008) 1002-1010. Yu L, Zhang B, Szilvay GR, Sun R, Jänis J, Cui Y, Feng S, Xu H, Linder MB & Qiao M: Protein HGFI from the edible mushroom Grifola frondosa is a novel 8 kDa class I hydrophobin that forms rodlets in compressed monolayers. Microbiology 154 (2008) 1677-1685. Määttä JAE, Airanne TT, Nordlund HR, Jänis J, Paldanius TA, Vainiotalo P, Johnson MS, Kulomaa MS & Hytönen VP: Rational modification of ligand-binding preference of avidin by circular permutation and mutagenesis. ChemBioChem 9 (2008) 1124-1135. Marjasvaara A, Jänis J & Vainiotalo P: Oxidation of a laccase mediator ABTS as studied by ESI-FTICR mass spectrometry, J. Mass Spectrom. 43 (2008) 470-477. 2007 PROTEIN CRYSTALLOGRAPHY
Niemi M, Jylhä S, Laukkanen M-L, Söderlund H, Mäkinen-Kiljunen S, Kallio JM, Hakulinen N, Haahtela T, Takkinen K & Rouvinen J: Molecular interactions between a recombinant IgE antibody and the b-lactoglobulin allergen. Structure, 15 (2007) 1413-1421.
Purmonen M, Valjakka J, Takkinen K, Laitinen T & Rouvinen J: Molecular dynamics studies on thermostability of family 11 xylanases. Prot. Eng. Des. Sel. 20 (2007) 551-559. Parkkinen T, Koivula A, Vehmaanperä J & Rouvinen J: Preliminary X-ray analysis of cellobiohydrolase Cel7B from Melanocarpus albomyces. Acta Cryst. F 63 754-757. Voutilainen SP, Boer H, Linder MB, Puranen T, Rouvinen J, Vehmaanperä J & Koivula
A: Heterologous expression of Melanocarpus albomyces cellobiohydrolase
Cel7B, and random mutagenesis and screening to improve its thermostability.
Enzyme Microbiol. Tech. 41 (2007) 234-243. 2007 PROTEIN MASS SPECTROMETRY
Jänis J, Pulkkinen P, Rouvinen J & Vainiotalo P: Determination of steady-state kinetic parameters for a xylanase-catalyzed hydrolysis of neutral underivatized xylooligosaccharides by mass spectrometry. Anal. Biochem. 365 (2007) 165-173. Helppolainen SH, Nurminen KP, Määttä JAE, Halling KK, Slotte PJ, Huhtala T, Liimatainen T, Ylä-Herttuala S, Airanne KJ, Närvänen A, Jänis J, Vainiotalo P, Valjakka J, Kulomaa MS & Nordlund HR: Rhizavidin from Rhizobium etli – the first natural dimer in the avidin protein family. Biochem. J. 405 (2007) 397–405. 2006 PROTEIN CYSTALLOGRAPHY
Parkkinen T, Nevanen
T, Koivula A & Rouvinen J: Crystal structures of an enantioselective
Fab and its complex with Finrozole. J. Mol. Biol. 357 (2006)
471-480.
Hakanpää J,
Linder M, Popov A, Schmidt A & Rouvinen J: Hydrophobin HFBII in
detail -ultrahigh resolution structure of 0.75 Å. Acta
Cryst. D 62 (2006) 356-367.
Hakanpää, J, Szilvay G, Kaljunen H, Maksimainen M, Linder
M & Rouvinen J: Two crystal structures of Trichoderma reesei hydrophobin
HFBI – a structure of a protein amphiphile with and without detergent. Protein
Sci. 15 (2006) 2129-2140. Teerinen T, Valjakka J, Rouvinen J & Takkinen K: Structure-based
stability engineering of mouse IgG1 Fab fragment by modifying constant
domains. J. Mol. Biol. 361 (2006) 687-697. Hakulinen N, Kruus K, Koivula A & Rouvinen J: A crystallographic
and spectroscopic study on the effect of X-ray radiation on the crystal
structure of Melanocarpus albomyces laccase. Biochem.
Biophys. Res. Comm. 350 (2006) 929-934. 2006 PROTEIN MASS SPECTROMETRY
Marjasvaara A, Torvinen M, Kinnunen H & Vainiotalo P: Laccase-catalyzed polymerization of two phenolic compounds studied by matrix-assisted laser desorption/ionization time-of-flight and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry with collision-induced dissociation experiments. Biomacromolecules 7 (2006) 1604.
Marjasvaara A, Kruus K & Vainiotalo, P: A laccase study by electrospray ionization Fourier transform ion cyclotron resonance MS: copper depletion, glycoforms and stability. J. Mass Spectrom. 41 (2006) 91.
2005 PROTEIN MASS SPECTROMETRY
Jänis J, Hakanpää J, Hakulinen N, Ibatullin FM, Hoxha
A, Derrick PJ, Rouvinen J & Vainiotalo P: Determination of thioxylooligosaccharide
binding to family 11 xylanases using electrospray fourier transform ion
cyclotron resonance mass spectrometry and X-ray crystallography. FEBS
J. 272 (2005) 2317-2333. 2004 PROTEIN CRYSTALLOGRAPHY
Palviainen J, Rouvinen
J & Silvennoinen R: Analysis of microfibril angle of wood fibers
by use of laser microscope polarimetry, Opt. Eng. 43 (2004)
186-191.
Hakanpää J,
Linder M, Askolin S, Nakari-Setälä T, Parkkinen T, Penttilä M & Rouvinen
J: Atomic resolution structure of the HFBII hydrophobin: a self-assembling
amphihile. J. Biol. Chem. 279 (2004) 527-533.
Hakanpää J,
Parkkinen T, Hakulinen N, Linder M & Rouvinen J: Crystallization
and preliminary X-ray characterization of Trichoderma reesei hydrophobin
HFBII. Acta Cryst. D 60 (2004) 163-165.
Simonaho
S-P, Tolonen Y, Rouvinen J & Silvennoinen R: Laser light scattering
from wood samples soaked in water and in benzyl bentsoate. Optik 114 (2004) 445-448.
Saarela J, Oinonen
C, Jalanko A, Rouvinen J & Peltonen L: Autoproteolytic processing
of aspartylglucosaminidase. Biochem J. 378 (2004) 363-371. Parkkinen T, Hakulinen
N, Tenkanen M, Siika-aho M & Rouvinen Juha:
Crystallization and preliminary X-ray analysis of a novel Trichoderma
reesei xylanase IV belonging to glycoside hydrolase family 5. Acta
Cryst. D. 60 542-544. Jokela, M, Eskelinen
A, Pospiech H, Rouvinen J & Syväoja JE:
Characterization of the 3´exonuclease subunit DP1 of Methanococcus
jannaschii replicative DNA polymerase D. Nucl. Acids Res. 32 (2004)
9556-9566. Karimäki J,
Parkkinen T, Santa S, Pastinen O, Leisola M, Rouvinen J & Turunen
O: Engineering the substrate specificity of xylose isomerase. Prot.
Eng. Des. Select. 17 (2004) 861-869. 2004 PROTEIN MASS SPECTROMETRY
Jänis J, Turunen O, Leisola M, Derrick PJ, Rouvinen J & Vainiotalo P: Characterization of mutant xylanases using Fourier tranform ion cyclotron resonance mass spectrometry: stabilizing contributions of disulphide bridgesand N-erminal extensions. Biochemistry 43 (2004)
9556-9566. A Marjasvaara, M Torvinen & Vainiotalo P: Laccase-catalyzed mediated oxidation of benzyl alcohol: the role of TEMPO and formation of products including benzonitrile studied by nanoESI-FTICR mass spectrometry. J. Mass Spectrom. 39 (2004) 1139-1146. Xiong H, Jänis J, Leisola M & Turunen O: Characterization of the xylanase produced by submerged cultivation of Thermomyces lanuginosus DSM 10635. Enzyme Microb. Technol. 35 (2004) 93-99. Turunen O, Jänis J, Fenel F & Leisola M: Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesis. Methods Enzymol. 388 (2004) 156-167. Fenel F, Leisola M, Jänis J & Turunen O: A de novo designed N-terminal disulphide bridge stabilizes the Trichoderma reesei endo-1,4-b-xylanase II. J. Biotechnol. 108 (2004) 137-143. 2003 PROTEIN CRYSTALLOGRAPHY
Nordman N, Valjakka
J & Peräkylä M:
Analysis of the binding energies of testosterone, 5a-dihydrotestosterone,
androstenedione abd
dehydroepiandrosterone sulfate with an antitestosterone antibody. Proteins 50 (2003)
135-143.
Valjakka J & Rouvinen
J: Crystal structure of 20k endoglucanase from Melanocarpus albomyces at
1.8 Å resolution. Acta
Cryst. D 59 (2003) 765-768.
Hakulinen N, Turunen
O, Jänis J, Leisola M & Rouvinen
J: Three-dimensional structures of thermophilic b-1,4-xylanases from Chaetomium thermophilum and Nonomuraea
flexuosa: Comparison of twelve xylanases in relation to their thermal
stability. Eur. J. Biochem. 270 (2003) 1399-1412.
Laitinen T, Rouvinen
J & Peräkylä M: MM–PBSA free
energy analysis of endo–1,4–xylanase II (XynII)–substrate
complexes: binding of the reactive sugar in a skew–boat and chair
conformation. Org. Biomolec. Chem. 1 (2003)
3535-3540.
2002 PROTEIN CRYSTALLOGRAPHY
Valjakka J, Takkinen
K, Söderlund H & Rouvinen
J: Structural insights to steroid hormone recognition and binding:
Crystal structure of a recombinant
anti-testosterone Fab-fragment in native and ligand bound form. J.
Biol. Chem. 277 (2002) 4183-4190.
Hakulinen N, Kiiskinen
L-L, Kruus K, Saloheimo M, Koivula A & Rouvinen
J: Crystal structure of a laccase from Melanocarpus albomyces with an
intact trinuclear copper site. Nature Struct. Biol. 9 (2002)
601-605.
Valjakka JM, Hemminki
A, Niemi S, Söderlund H, Takkinen K & Rouvinen
J: Crystal structure of an in vitro affinity and specificity-matured
anti-testosterone Fab in complex with testosterone. Improved affinity
results from small structural changes within the variable domains. J.
Biol. Chem. 277 (2002) 44021-44027.
2002 PROTEIN MASS SPECTROMETRY
Nousiainen M, Derrick PJ, Kaartinen, MT, Mäenpää PH,
Rouvinen J & Vainiotalo P: The mass spectrometric study of metal binding to osteocalcin. Chem. Biol. 9 (2002) 195-202.
Nousiainen M, Cooper HJ, Fati D, Hoxha A, Trayer HR, Ward DG, Vainiotalo P, Derrick PJ & Trayer IP: Calcium and peptide binding to folded and unfolded conformations of cardiac troponin C. Electrospray ionization and fourier transform ion cyclotron resonance mass spectrometry. Eur. J. Mass Spectrom. 8 (2002) 471.
2001 PROTEIN CRYSTALLOGRAPHY
Mäenpää PH, Väisänen S, Jääskeläinen
T, Ryhänen S, Rouvinen J, Duchier C, Mahonen A: Vitamin D(3) analogs
(MC 1288, KH 1060, EB 1089, GS 1558, and CB 1093): studies on their mechanism
of action. Steroids 66 (2001) 223-225.
Rouvinen J, Virtanen
T & Mäntyjärvi
R: Searching for the determinants of allergenicity in proteins of the
lipocalin family. J.
Chromatogr. B. 756(2001) 199-206.
Turunen O, Etuaho
K, Fenel F, Rouvinen J, Vehmaanperä J & Leisola
M: Combination of several mutations in Trichoderma reesei XYNII has
a cumulative effect on the termal stability. J. Biotech. 88 (2001)
37-46.
Saarela J, Laine
M, Oinonen C, Jalanko A, Rouvinen J & Peltonen L:
Characterization of molecular pathogenesis of a disease: Structural consequences
of aspartylglucosaminuria mutations. Human Mol. Genet.10 (2001)
983-995.
2001 PROTEIN MASS SPECTROMETRY
Jänis J, Rouvinen J, Leisola M, Turunen O & Vainiotalo
P: Thermostability of endo-1,4-beta-xylanase II from Trichoderma reesei
studied by electrospray ionization Fourier-transform ion cyclotron resonance
MS, hydrogen/deuterium-exchange reactions and dynamic light scattering. Biochem
J.356 (2001) 453-460.
Nousiainen M, Vainiotalo P, Feng X & Derrick PJ: Calmodulin-RS20-Ca4 complex in the gas phase: electrospray ionisation and Fourier transform ion cyclotron resonance. Eur. J. Mass Spectrom. 7 (2001) 393.
2000 PROTEIN CRYSTALLOGRAPHY
Valjakka J, Hemminki
A, Teerinen T, Takkinen K & Rouvinen J: X-ray studies
of recombinant anti-testosterone Fab-fragments: the use of PEG 3350 in
crystallization. Acta Cryst. D. 56(2000) 218-221.
Hakulinen N, Paavilainen
S, Korpela T & Rouvinen J: The Crystal Structure
of beta-Glucosidase from Bacillus circulans sp. alkalophilus: ability
to form long polymeric assemblies. J. Struct. Biol. 129 (2000)
69-79.
Laitinen T, Rouvinen
J & Peräkylä M:
Inversion of the roles of the nucleophile and acid/base catalysts in
the covalent binding of
epoxyalkyl xyloside inhibitor to the catalytic glutamates of endo-1,4-b-xylanase
(XYNII): a molecular dynamics study. Protein Eng. 13 (2000)
247-252.
Kerovuo J, Rouvinen
J & Hatzack
F: Analysis of myo-inositol hexakisphosphate hydrolysis by Bacillus
phytase: indication of a novel reaction mechanism. Biochem.
J. 352 (2000) 623-628.
Oinonen C & Rouvinen
J: Structural comparison of Ntn-hydrolases. Protein
Sci. 9 (2000) 2329-2337.
Hakulinen N, Tenkanen
M & Rouvinen
J: The crystal structure of the catalytic core of acetyl xylan esterase
from Trichoderma reesei. J.
Struct. Biol. 132 (2000) 180-190.
1999 PROTEIN CRYSTALLOGRAPHY
Rouvinen J, Rautiainen
J, Virtanen, T, Zeller T, Kauppinen J, Taivainen A & Mäntyjärvi
R: Probing the molecular basis of allergy. Three-dimensional structure
of major bovine allergen Bos d 2. J. Biol. Chem. 274 (1999)
2337-2343.
Väisänen S, Rouvinen J & Mäenpää PH:
The importance of the putative helices 4 and 5 of human vitamin D receptor
for ligand
binding. Biochem. Biophys. Res. Comm. 264 (1999) 478-482.
1998 PROTEIN CRYSTALLOGRAPHY
Hakulinen N, Tenkanen
M & Rouvinen
J: Crystallization and preliminary X-ray diffraction studies of the
catalytic core of acetyl xylan esterase
from Trichoderma reesei, Acta Cryst. D54 (1998) 430-432.
Koivula A., Kinnari
T, Harjunpää V, Ruohonen L, Teleman A,
Drakenberg, T, Rouvinen J, Jones TA & Teeri TT: Tryptophan 272: an essential
determinant of crystalline cellulose degradation by Trichoderma reesei cellobiohydrolase
Cel6A. FEBS Lett. 429 (1998) 341-346.
Kyngäs J & Valjakka
J: Unreliability of the Chou-Fasman parameters in predicting protein
secondary structure. Protein Eng. 11 (1998)
345-348.
Muilu J, Törrönen A, Peräkylä M & Rouvinen
J: Conformational changes and the catalytic cycle of endo-1,4-xylanase
II from Trichoderma
reesei: a molecular dynamics study. Proteins 31 (1998)
434-444.
Rautiainen J, Auriola
S, Rouvinen J, Kauppinen J, Zeiler T, Novikov D, Virtanen T &. Mäntyjärvi
RA: Molecular and crystal properties of Bos d 2, an allergenic protein
of the lipocalin family. Biochem.
Biophys. Res. Comm. 247 (1998) 746-750.
Saarela J, Peltola
M, Tikkanen R, Oinonen C, Jalanko A, Rouvinen J & Peltonen
L: Activation and oligomerization of aspartylglucosaminidase, J. Biol.
Chem. 273 (1998) 25320-25328.
Laitinen T, Rouvinen
J & Peräkylä M:
Ab initio quantum mechanical and density functional theory calculations
of nucleophile- and nucleophile
and acid-catalyzed opening of an epoxide ring: A model for the covalent
binding of epoxyalkyl inhibitors to the active site of glycosidases. J.
Org. Chem. 63 (1998) 8157-8162.
Quack M, Szafranski
K, Rouvinen J & Carlberg C: The role of the T-box
for the function of the vitamin D receptor on different types of response
elements. Nucl. Acids Res. 26 (1998) 5372-5378.
Väisänen S, Rouvinen J & Mäenpää PH:
Putative helices 3 and 5 of the human vitamin D3 receptor are important
for the
binding of calcitriol. FEBS Lett.440(1998) 203-207.
1998 PROTEIN MASS SPECTROMETRY
Nousiainen M, Rafn K, Skou L, Roepstorff P & Andersen SO: Characterization of exoskeletal proteins from the american lobster, Homarus americanus. Comp. Biochem. Physiol. 119 (1998) 189.
1997 PROTEIN CRYSTALLOGRAPHY
Törrönen A & Rouvinen
J: Structural and functional properties of low molecular weight endo-1,4-b-xylanases, J. Biotech. 57 (1997)
137-149.
Tikkanen R, Peltola
M, Oinonen C, Rouvinen J & Peltonen L: Several
cooperating binding sites mediate the interaction of a lysosomal enzyme
with phosphotransferase, EMBO J. 16 (1997) 6684-6693.
1996 PROTEIN CRYSTALLOGRAPHY
Tikkanen R, Rouvinen
J, Törrönen A, Kalkkinen N & Peltonen
L: Large-Scale Purification and Preliminary X-ray Diffraction Studies
of Human Aspartylglucosaminidase, Proteins24(1996) 253-258.
Tikkanen R, Riikonen
A, Oinonen C, Rouvinen J & Peltonen L: Functional
analyses of active site residues of human lysosomal aspartylglucosaminidase:
implications for catalytic mechanism and autocatalytic activation, EMBO
J.15(1996) 2954-2960.
Havukainen R, Törrönen A, Laitinen T & Rouvinen
J: Covalent Binding of Three Epoxyalkyl-Xylosides to the Active Site
of Endo-1,4-Xylanase
II from Trichoderma reesei, Biochemistry35(1996) 9617-9624.
Riikonen A, Rouvinen
J, Tikkanen R, Julkunen I, Peltonen L & Jalanko
A: Primary folding of aspartylglucosaminidase. Significance of disulphide
bridges and evidence for early multimerization, J. Biol. Chem. 271 (1996)
21340-21344.
Koivula A, Reinikainen
T, Ruohonen L, Valkeajärvi A, Claeyssens
M, Teleman O, Kleywegt GJ, Szardenings M, Rouvinen J, Jones TA & Teeri
TT: The active site of Trichoderma reesei cellobiohydrolase II:
the role of tyrosine 169, Protein Eng. 9(1996) 691-699.
Kyngäs J & Valjakka
J: Evolutionary Neural Networks in Quantitative Structure-Activity
Relationships of Dihydrofolate Reductase Inhibitors, Quant.
Struct.-Act. Relat. 15 (1996) 296-301
Peräkylä M & Rouvinen
J: Ab Initio Quantum Mechanical
Study on the Catalytic Mechanism of Aspartylglucosaminidase (AGA): A
Serine Protease-like Mechanism with an N-terminal Threonine and Substrate-Assisted
Catalysis, Chem. Eur. J. 2 (1996) 1548-1551.
1995 PROTEIN CRYSTALLOGRAPHY
Törrönen A & Rouvinen
J: Structural comparison of two major endo-1,4-b-xylanases from Trichoderma reesei, Biochemistry 34 (1995)
847-856.
Oinonen C, Tikkanen
R, Rouvinen J & Peltonen L: Three-dimensional structure
of human lysosomal aspartylglucosaminidase, Nature Struct. Biol. 2 (1995)
1102-1108.
1994 PROTEIN CRYSTALLOGRAPHY
Silvennoinen R, Nygren
K, Rouvinen J & Petrova V: Bone structure studies
with HNDT and X-ray diffraction methods, Opt. Eng. 33 (1994)
473-478.
Törrönen A, Harkki A & Rouvinen
J: Crystallographic studies on endo-1,4-b-xylanase II from Trichoderma reesei: two conformational
states in the active site, EMBO J. 13 (1994) 2493-2501.
Luo Z, Rouvinen
J & Mäenpää PH:
A peptide C-terminal to the second Zn finger of human vitamin D receptor
is able to specify
nuclear localization, Eur. J. Biochem. 223 (1994) 381-387.
1993 PROTEIN CRYSTALLOGRAPHY
Törrönen A, Rouvinen J, Ahlgren M, Harkki A & Visuri
K: Crystallization and Preliminary X-ray Analysis of Two Major Xylanases
from Trichoderma
reesei, J. Mol. Biol. 233 (1993) 313-316.
Rouvinen J, Hoffren
AM, Pakkanen TA & Karjalainen A: Modelling of a2-adrenergic
drugs, Drug Design and Discovery 10 (1993) 285-295.
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