structural biology

department of chemistry biocenter
Kuopio   		kemian
laitos  
 

MS-INSTRUMENTATION

HIGH-RESOLUTION ESI Q-FT-ICR MASS SPECTROMETER

  • Bruker APEX-Qe hybrid quadrupole-Fourier transform ion cyclotron resonance (Q-FT-ICR) mass spectrometer (Bruker Daltonics)
  • Continuous flow electrospray ionization (ESI) with Apollo-II ion source (Bruker)
  • On-line/off-line nanoelectrospray (NanoESI) options
  • Passively-shielded 4.7-tesla superconducting magnet (Magnex Scientific)
  • A variety of tandem mass spectrometric methods available (CID, SORI-CID, IRMPD, ECD, EID)
  • Special inlet/pulsed valves for reagent introduction for gas-phase ion/molecule reactions
  • High dynamic range (m/z 150-10000)
  • High mass resolving power (> 250.000) and mass accuracy (ppb)
  • High sensitivity (nM)

ESI QIT MASS SPECTROMETER

  • Bruker ESQUIRE-3000 Plus quadrupole ion trap (QIT) mass spectrometer
  • Continuous flow electrospray ionization (ESI)
  • Dynamic range m/z 50-3000 (up to m/z 6000 in extended mode)
  • Mass resolving power up to 0.25 FWHM in high-resolution mode
  • High sensitivity (nM)
  • A multiple-stage tandem mass spectrometry (CID) up to MS10